Laccase production by newly isolated white rot fungus Funalia trogii: Effect of immobilization matrix on laccase production

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• 作者：Emre Birhanli^a ; Selim Erdogan^b ; Ozfer Yesilada^a ; ^{ozfer.yesilada@inonu.edu.tr} ; Yunus Onal^c
• 关键词：Activated carbon ; Enzymes ; Enzyme production ; Fermentation ; Filamentous fungi ; Laccase
• 刊名：Biochemical Engineering Journal
• 出版年：2013
• 出版时间：15 February, 2013
• 年：2013
• 卷：71
• 期：Complete
• 页码：134-139
• 全文大小：596 K

文摘

The laccase production performance of newly isolated Funalia trogii (F. trogii) immobilized on low-cost activated adsorbents prepared from apricot stone was investigated in inexpensive molasses medium and compared with free pellets. The adsorbents were apricot stone-based activated carbon (AC), Cu-impregnated apricot stone-based activated carbon (Cu-AC) and Fe-impregnated apricot stone-based activated carbon (Fe-AC). Cu-AC and Fe-AC were prepared by chemical activation. The values of BET surface area (S_BET), total pore volume (V_t), micropore surface area (S_mic) and micropore volume (V_mic) which show the pore properties of the activated carbons were 133 m² g^?1, 0.105 cm³ g^?1, 108 m² g^?1 and 0.056 cm³ g^?1 for Cu-AC and 145 m² g^?1, 0.112 cm³ g^?1, 117 m² g^?1 and 0.061 cm³ g^?1 for Fe-AC, respectively. F. trogii attached well on all of these adsorbents. However, the fungus immobilized on Cu-AC produced much higher levels of laccase than the others. The laccase activity obtained in 5 g L^?1 molasses media after first cycle and mean laccase activity obtained after three cycles with fungus immobilized on Cu-AC were 29.23 U mL^?1 and 27.04 U mL^?1, respectively. These activities were only 0.25 and 0.39 U mL^?1 with free pellets. The crude laccase from immobilized culture could also decolorize the textile dyes.