Antibodies against neo-epitope tTg complexed to gliadin are different and more reliable then anti-tTg for the diagnosis of pediatric celiac disease

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• 作者：Aaron Lerner^a ; ^b ; ^{aaronlerner1948@gmail.com" class="auth_mail" title="E-mail the corresponding author} ; Patricia Jeremias^a ; Sandra Neidhö ; fer^a ; Torsten Matthias^a ; ^{matthias@aesku.com" class="auth_mail" title="E-mail the corresponding author}
• 关键词：tTg ; tissue transglutaminase ; tTg-neo ; tTg complexed to gliadin ; PCD ; pediatric celiac disease ; AP ; abdominal pain ; NC ; normal children ; NA ; normal adults ; RA ; rheumatoid arthritis ; CD ; celiac disease ; ESPGHAN ; European Society of Pediatric Gastroenterology ; Hepatology and Nutrition
• 刊名：Journal of Immunological Methods
• 出版年：2016
• 出版时间：February 2016
• 年：2016
• 卷：429
• 期：Complete
• 页码：15-20
• 全文大小：735 K

文摘

The neo-epitope tTg (tTg-neo) autoantibody, never challenged the anti-tissue transglutaminase (tTg) premiership, recommended by ESPGHAN, for celiac disease (CD) diagnosis.

d="sp0040">Pediatric CD (PCD), abdominal pains and normal children, normal adults, and rheumatoid arthritis patients, were tested using the following ELISAs detecting IgA, IgG or both IgA and IgG (check): AESKULISA® tTg (tTg; RUO) and AESKULISA® tTg-neo.

d="sp0045">Higher OD activity was detected for tTg-neo IgA, IgG and IgA + IgG than for tTg. tTg-neo IgA, IgG correlated better with intestinal damage than tTg. The tTg-neo combined IgA + IgG ELISA kit had higher sensitivity and a comparable specificity for the diagnosis of PCD. The drop in the % competition was much higher with the tTg-neo then the tTg antibodies. The false positivity of the tTg was significantly higher than the tTg-neo one.

d="sp0050">Serological diagnostic performances, reflection of intestinal damage, diverse epitopes and false positivity were better with the tTg-neo.