Regulation of actin filament turnover by cofilin-1 and cytoplasmic tropomyosin isoforms

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• 作者：Zofia Ostrowska ; Katarzyna Robaszkiewicz ; Joanna Moraczewska ; ^{joanna.moraczewska@ukw.edu.pl}
• 关键词：ADF ; actin depolymerizing factor ; F-actin ; polymerized actin ; HMM ; heavy meromyosin ; SDS-PAGE ; polyacrylamide gel electrophoresis in SDS ; DTT ; dithiothreitiol ; MW ; molecular weight ; LMW ; low molecular weight ; HMW ; high-molecular weight ; TPM ; tropomyosin encoding gene ; Tpm ; tropomyosin ; TRC ; N(-tetramethylrhodaminyl) cadaverine
• 刊名：Biochimica et Biophysica Acta (BBA) - Proteins and Proteomics
• 出版年：2017
• 出版时间：January 2017
• 年：2017
• 卷：1865
• 期：1
• 页码：88-98
• 全文大小：1580 K
• 卷排序：1865

文摘

Cytoskeletal tropomyosin isoforms and cofilin-1 diversify dynamics of microfilaments. Cofilin-1 ejects tropomyosin isoforms from microfilaments with different efficiency. Tpm1.6/1.8 slow down, but Tpm3.2/3.4 accelerate cofilin-induced depolymerization. Tpm3.2/3.4 enhance cofilin-induced release of phalloidin from actin filaments. Actin affinity and binding cooperativity underlie the regulatory mechanisms.