Mitochondrial superoxide reduction and cytokine secretion skewing by carbon nanotube scaffolds enhance ex vivo expansion of human cord blood hematopoietic progenitors

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• 作者：Sudipto Bari ; BEng^a ; ^h ; Pat Pak Yan Chu ; PhD^b ; Andrea Lim ; BSc^c ; Xiubo Fan ; PhD^d ; Ralph Milford Bunte ; DVM^e ; Shang Li ; PhD^f ; Sujoy Ghosh ; PhD^c ; ^g ; Gigi Ngar Chee Chiu ; PhD^a ; ^{phacncg@nus.edu.sg" class="auth_mail" title="E-mail the corresponding author} ; William Ying Khee Hwang ; FRCP ; FAMS ; MMed ; MRCP ; MBBS^b ; ^f ; ^h ; ^{william.hwang.y.k@sgh.com.sg" class="auth_mail" title="E-mail the corresponding author}
• 关键词：Ex vivo expansion of UCB ; Surface functionalized single walled carbon nanotubes (f-SWNCT) ; Hematopoietic stem cell transplantation (HSCT) ; Immunodeficient mouse model ; Umbilical cord blood (UCB)
• 刊名：Nanomedicine: Nanotechnology, Biology and Medicine
• 出版年：2015
• 出版时间：October 2015
• 年：2015
• 卷：11
• 期：7
• 页码：1643-1656
• 全文大小：2143 K

文摘

In this study, we report that surface functional groups of single walled carbon nanotubes (f-SWCNT) are critical for mediating survival and ex vivo expansion of hematopoietic stem and progenitor cells (HSPC) in human umbilical cord blood (UCB). In comparison to amide (− O-NH₂) and polyethylene-glycol (− PEG) functionalized SWCNT, carboxylic acid (− COOH) variants gave optimal viability support which correlated with maximal reduction of lethal mitochondrial superoxides in HSPC. Cytokine array illustrated that f-SWCNT-COOH maintained higher proportion of HSPC associated cytokines and minimal level of differentiation promoting factors. Transplantation of f-SWCNT-COOH expanded grafts in sub-lethally irradiated immunodeficient mice resulted in higher engraftment of HSPC in bone marrow compared to control when they were co-transplanted with non-expanded cells from the same UCB. Expanded grafts mediated higher survival rate of mice compared to non-expanded grafts due to lower graft-versus-host-disease which is likely a consequence of proportion of immune cells in the grafts.

From the Clinical Editor

Umbilical cord blood (UCB) is a potential source of hematopoietic stem and progenitor (HSPC) cells. One major hurdle for its clinical use is the insufficient yield of cell number. The authors in this study elegantly demonstrated the importance of various functional groups on single-walled carbon nanotubes (f-SWCNT) in enhancing ex vivo expansion of HSPC in UCB. The findings may pave a way for having UCB as a source for HSPC for clinical use in the future.