Melanocytes in the corneal limbus interact with K19-positive basal epithelial cells

详细信息    查看全文

• 作者：Kazunari Higa ; Shigeto Shimmura ; Hideyuki Miyashita ; Jun Shimazaki and Kazuo Tsubota
• 关键词：melanin ; melanocyte ; corneal epithelium ; keratin ; immunohistochemistry
• 刊名：Experimental Eye Research
• 出版年：2005
• 出版时间：2005
• 年：2005
• 卷：81
• 期：2
• 页码：218-223
• 全文大小：288 K

文摘

The human corneal limbus is identified by the distinct features of the palisades of Vogt (POV), which contain pigment granules that are aligned with the microplicae of the epithelium. Although it is presumed that pigments are produced by melanocytes, the characterization of melanocytes in the limbus has not been clearly documented. We examined human limbal tissues by whole mounts and serial histological sections to localize epithelial cells containing melanin granules. Most of the pigmented cells observed by immunohistochemistry were K19 (+) cells in the basal limbal epithelium. A superimposed image revealed that melanin granules were oriented towards the apex of each K19 (+) cell, acting as a pigmented cap facing the ocular surface. Melanocytes were identified by MART1, an antigen specific to melanocyte-lineage cells. Melanocytes were shown to exist as sporadic cells with dendritic processes that extend to surrounding epithelial cells. Melanocytes were also found in light-pigmented donor tissue when visualized by the tyrosinase assay using the enzyme substrate DOPA. Since tyrosinase activity was not found in epithelial cells, the production of melanin is exclusively the role of melanocytes that comprised 5·3±2·7 % of the total cells in cytospin samples (N