Increased expression of oxidative enzymes in adipose tissue following PPAR伪-activation

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• 作者：Adaliene Versiani Matos Ferreira^a ; ^{adaliene@gmail.com" class="auth_mail} ; Zé ; lia Menezes-Garcia^b ; Erica Guilhen Mario^c ; Helen Lima Delpuerto^c ; Almir Souza Martins^c ; Leida Maria Botion^c
• 关键词：ACO ; Acyl CoA Oxidase ; EAT ; Epididymal adipose tissue ; CPT-1 ; Carnitine palmitoyltransferase 1 ; FFA ; free fatty acids ; PPARs ; peroxisome proliferator-activated receptors
• 刊名：Metabolism
• 出版年：April, 2014
• 年：2014
• 卷：63
• 期：4
• 页码：456-460
• 全文大小：281 K

文摘

Objective

Evaluate the effect of fenofibrate treatment on the expression of PPAR伪 and oxidative enzymes in adipose tissue.

Materials/methods

Wistar male rats were fed a balanced diet supplemented with 100 mg.Kg-1 bw.day-1 fenofibrate (Sigma) during nine days. Plasma glucose, free fatty acids (FFA) leptin and insulin were determined. PPAR伪, ACO and CPT-1 mRNA expression and amount of PPAR伪 and PPAR纬 protein were assessed in epididymal adipose tissue. Oral glucose tolerance test was evaluated into overnight fasted rats. Glucose uptake was measured in adipocytes isolated from epididymal fat pads in the presence or absence of insulin (25 ng/mL).

Results

Fenofibrate treatment increased PPAR伪 and PPAR纬 protein abundance in adipose tissue. In addition to it well- known effect on oxidative enzymes in liver, fenofibrate treatment also induces a high expression of Acyl CoA Oxidase (ACO) and Carnitine palmitoyltransferase 1 (CPT-1) in adipose tissue. Furthermore, we have shown that the fenofibrate treatment improves the glucose tolerance and enhance the glucose uptake by adipocytes.

Conclusion

Altogether, the data suggest that fenofibrate have a direct effect in adipose tissue contributing to the low adiposity and improvement of glucose homeostasis.