Determining the absolute abundance of dinoflagellate cysts in recent marine sediments III: Identifying the source of Lycopodium loss during palynological processing and further testing of the Lycopodium marker-grain method
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Cyst/Lycopodium spore ratios between 0.5 and 2.0 are recommended to facilitate counting.

Using powdered sediment and sonicating for more than 5 min is not recommended.

The largest loss of cysts and Lycopodium spores occurs during sieving.

This loss leads to overestimates in calculated cyst concentrations of only ~ 4.8%

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