Pullulan as a linear carbohydrate was utilized as liver targeting molecules and conjugated to polyamidoamine (PAMAM). PAMAM-pullulan derivatives exhibited better transfection efficiency than the unmodified PAMAM and pullulan with reduced cytotoxicity in hepatocyte cells. Delivery of nucleic acids into the non-targeted cells was similar to unmodified PAMAM confirming the targeting property of pullulan.