Dynamic metabolic profile of Zhi-Zi-Da-Huang decoction in rat urine based on hybrid liquid chromatography-mass spectrometry coupled with solid phase extraction
详细信息 查看全文
- 作者：Juanjuan Wanga ; b ; Qingshui Shic ; Chunyong Wua ; b ; analysis99@126.com" class="auth_mail" title="E-mail the corresponding author ; Fang Fenga ; b ; fengfang1@hotmail.com" class="auth_mail" title="E-mail the corresponding author ; fengfang1@126.com" class="auth_mail" title="E-mail the corresponding author
- 关键词：DAD ; diode array detector ; m/z ; mass-to-charge ratio ; SPE ; solid phase extraction ; ZZDHD ; Zhi-Zi-Da-Huang decoction ; LLE ; Liquid-liquid extraction ; PPT ; protein precipitation ; TCM ; traditional Chinese medicine ; HPLC&ndash ; TOF/MS ; High performance liquid chromatography tandem time-of-flight mass spectrometry ; HPLC&ndash ; QqQ/MS ; High performance liquid chromatography tandem triple quadrupole mass spectrometry
- 刊名：Journal of Chromatography B, Analytical Technologies in the Biomedical and Life Sciences
- 出版年：2016
- 出版时间：15 November 2016
- 年：2016
- 卷：1036-1037
- 期：Complete
- 页码：100-113
- 全文大小：2521 K
文摘
- •
An optimized solid phase extraction method was built to concentrate and purify the analytes in rat urine.
- •
HPLC–DAD–ESI–TOF/MS combined with HPLC–ESI–QqQ/MS was adopted to separate and identify the metabolites of Zhi-Zi-Da-Huang decoction in vivo.
- •
25 parent compounds and 28 metabolites, especially two new metabolites were identified.
- •
Urine samples collected at different time period were screened to investigate the dynamic metabolic process of Zhi-Zi-Da-Huang decoction in vivo.