Panax ginseng polysaccharide suppresses metastasis via modulating Twist expression in gastric cancer

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• 作者：Jian-Ping Cai^a ; ¹ ; Yang-Jiazi Wu^b ; ¹ ; Cong Li^a ; Mei-Yan Feng^a ; Qing-Tao Shi^a ; Rui Li^d ; Zhen-Yu Wang^e ; Jing-Shu Geng^a ; ^c ; ^{jingshugeng@126.com}
• 关键词：Panax ginseng ; Polysaccharide ; Twist ; Gastric cancer ; Metastasis ; Epithelial&ndash ; mesenchymal transition (EMT)
• 刊名：International Journal of Biological Macromolecules
• 出版年：2013
• 出版时间：June, 2013
• 年：2013
• 卷：57
• 期：Complete
• 页码：22-25
• 全文大小：607 K

文摘

It was previously reported that an antitumor polysaccharide (PGPW1) was isolated from the root of Panax ginseng. To extend our study, we investigated here the anti-invasive and metastatic effects of PGPW1 on human gastric cancer cell line HGC-27 and tried to determine its possible mechanism of action. Both scratch wound-healing and Transwell assay identified that PGPW1 dose-dependently inhibited migration and invasiveness of HGC-27 cells. Furthermore, results of western blot showed that protein levels of Twist and AKR1C2 were inhibited by PGPW1, whereas an increase of NF1 was observed. Moreover, down-regulation of Twist expression by PGPW1 blocked epithelial-mesenchymal transition (EMT), characterized by a gain of epithelial cell markers, E-cadherin, and loss of the mesenchymal markers, vimentin and N-cadherin, at protein levels. Collectively, we confirmed that PGPW1 decreased migration and invasion of HGC-27 cells by regulation of Twist, AKR1C2, NF1, E-cadherin, vimentin and N-cadherin expression. In conclusion, PGPW1 may serve as a powerful chemopreventive agent against gastric cancer metastasis.