Development of fluorescence-conjugated islet-homing peptide using biopanning for targeted optical imaging of pancreatic islet

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• 作者：Min Jun Kim^a ; ¹ ; Jeong Heon Yu^b ; ¹ ; Mi Hwa Oh^c ; Yoon Sung Nam^b ; ² ; ^{yoonsung@kaist.ac.kr} ; Dong Yun Lee^a ; ² ; ^{dongyunlee@hanyang.ac.kr}
• 关键词：Islet-homing peptide ; Pancreatic islet ; Phage display ; Biopanning ; Islet imaging
• 刊名：Journal of Industrial and Engineering Chemistry
• 出版年：2017
• 出版时间：25 January 2017
• 年：2017
• 卷：45
• 期：Complete
• 页码：404-411
• 全文大小：2091 K
• 卷排序：45

文摘

Visualization of pancreatic islet can provide important information for diagnosis and monitoring of pancreatic islets. By using phage display technology, here we newly identified four candidate peptide sequences with islet-homing property. In vitro binding assay showed that LSALPRT peptide had stronger affinity with islet cells than other candidates. Then this islet-homing peptide (IHP) was chemically conjugated with TAMRA fluorophore for near infrared (NIR) imaging of islet cells. This TMARA-IHP was strongly bound to islets in vitro, but also to cryo-sectioned slice of pancreas tissue ex vivo. Collectively, it would be a considerable potential as therapeutics or diagnostics in future studies.