Investigation of the structural organization of cationic nanoemulsion/antisense oligonucleotide complexes

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• 作者：Fern ; a Bruxel ; Jos茅 Mario Carneiro Vilela ; Margareth Spangler Andrade ; 脗ngelo Malachias ; Carlos A. Perez ; Rog茅rio Magalh茫es-Paniago ; M么nica Cristina Oliveira ; Helder F. Teixeira
• 关键词：Antisense oligonucleotides ; Atomic force microscopy ; Cationic lipids ; Nanoemulsions ; Energy dispersive X-ray diffraction ; Phospholipids ; X-ray diffractometry
• 刊名：Colloids and Surfaces B: Biointerfaces
• 出版年：1 December, 2013
• 年：2013
• 卷：112
• 期：Complete
• 页码：530-536
• 全文大小：1376 K

文摘

Atomic force microscopy image analysis and energy dispersive X-ray diffraction experiments were used to investigate the structural organization of cationic nanoemulsion/oligonucleotide complexes. Oligonucleotides targeting topoisomerase II gene were adsorbed on cationic nanoemulsions obtained by means of spontaneous emulsification procedure. Topographical analysis by atomic force microscopy allowed the observation of the nanoemulsion/oligonucleotide complexes through three-dimensional high-resolution images. Flattening of the oil droplets was observed, which was reduced in the complexes obtained at high amount of adsorbed oligonucleotides. In such conditions, complexes exhibit droplet size in the 600 nm range. The oligonucleotides molecules were detected on the surface of the droplets, preventing their fusion during aggregation. A lamellar structure organization was identified by energy dispersive X-ray diffraction experiments. The presence of the nucleic acid molecules led to a disorganization of the lipid arrangement and an expansion in the lattice spacing, which was proportional to the amount of oligonucleotides added.