The culture of gut explants: A model to study the mucosal response

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• 作者：Ilaria Russo ; Pio Zeppa ; Paola Iovino ; Chiara Del Giorno ; Fabiana Zingone ; Cristina Bucci ; Alessandro Puzziello ; Carolina Ciacci ; ^{cciacci@unisa.it" class="auth_mail" title="E-mail the corresponding author}
• 关键词：Challenge ; Gut explants ; In vitro culture response ; Inflammation ; Model of study
• 刊名：Journal of Immunological Methods
• 出版年：2016
• 出版时间：November 2016
• 年：2016
• 卷：438
• 期：Complete
• 页码：1-10
• 全文大小：5293 K

文摘

Various experimental model designs have been used to analyze the inflammatory pathways in human gastrointestinal illnesses. Traditionally, analytical techniques and animal models are popular experimental tools to study the inflammation process of intestinal diseases. However, the comparison of results between animal and human models is difficult for the inconsistency of outcomes. Although there are different animal models for studying the intestinal diseases, none of them fully represents the physiological and environmental conditions typical of the human species. Also, there is currently a concerted effort to decrease the experimental use of animals. On the converse, experimental protocols using the culture of gut mucosa had become popular with the advent of endoscopy which allows explanting multiple fragments from the intestine. The peculiar characteristic of this model is the ability to preserve in vitro the features that we found in vivo, thus also the response to various stimuli that differs from person to person.

The aim of the present paper is to provide a review of some of the possible uses of the organ intestinal mucosa culture.