Regulation of inhibin/activin expression in rat early antral follicles

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• 作者：Luz Andreone ; Ethel V. Velá ; squez ; Dalhia Abramovich ; Ver&#xf3 ; nica Ambao ; Nazareth Loreti ; Horacio B. Croxatto ; Fern ; a Parborell ; Marta Tesone ; Stella Campo
• 关键词：Early antral follicles ; Inhibin ; Activin ; 25-Hydroxycholesterol
• 刊名：Molecular and Cellular Endocrinology
• 出版年：2009
• 出版时间：15 October 2009
• 年：2009
• 卷：309
• 期：1-2
• 页码：48-54
• 全文大小：553 K

文摘

The aim of the present study was to determine the endocrine activity of cultured early antral follicles (EAF) isolated from prepubertal diethylstilbestrol-treated rats. The effect of steroidogenic substrates and FSH on steroid, inhibin A and B, Pro-αC and activin A production was evaluated. Androsterone was the predominant steroid produced by EAF. The addition of androstenedione, androstenedione + FSH and progesterone stimulated oestradiol production, whereas 25-hydroxycholesterol (25-OH-Chol) increased progesterone production. Inhibin A, B, Pro-αC, and activin A were produced under basal conditions. The predominance of inhibin B over inhibin A was not affected by the addition of androstenedione or progesterone. Inhibin A and activin A production was stimulated by FSH. 25-OH-Chol increased Inha, Inhba and Inhbb mRNA expression and the production of the three molecular forms of inhibins but decreased activin A production. These results show that FSH and the steroid follicular microenvironment differentially modulate the gene expression of inhibin/activin subunits, their assembly and secretion.