- 作者：Dalhia Abramovich ; Griselda Irusta ; Fern ; a Parborell ; Marta Tesone
- 关键词：Ovary ; VEGFA ; angiogenesis ; proliferation ; apoptosis ; PI3K/AKT
- 刊名：Fertility and Sterility
- 出版年：2010
- 出版时间：15 March 2010
- 年：2010
- 卷：93
- 期：5
- 页码：1369-1377
- 全文大小：813 K
Objective
To investigate the effects of local inhibition of vascular endothelial growth factor A (VEGFA) on proliferation and apoptosis of follicular cells in rat ovaries. To analyze the role of the PI3K/AKT signaling pathway on VEGFA effects.Design
Experimental study.
Setting
Research laboratory.
Animal(s)
Female Sprague Dawley rats, 21 days old, treated with equine chorionic gonadotropin (eCG).
Main Outcome Measure(s)
Follicular cell proliferation, apoptosis, and activation of the PI3K/AKT signaling pathway after intrabursal injection of a VEGFA inhibitor.
Result(s)
Inhibition of VEGFA leads to a decrease in the expression of the proliferation marker proliferating cell nuclear antigen (PCNA) in theca and granulosa cells (GC) and an increase in the activation of caspase 3 in antral follicles. Furthermore, we observed a decrease in the phosphorylation of RAC-alpha serine/threonine-protein kinase (AKT) and its target Bcl2 antagonist of cell death (BAD). No differences were found in the levels of kinase insert domain receptor (KDR) protein or in endothelial cell density.
Conclusion(s)
The VEGFA prevents apoptosis and stimulates proliferation of follicular cells, regulating follicular growth and development. The PI3K/AKT signaling pathway is one of the pathways involved in this mechanism. Therefore, VEGFA has a role as an antiapoptotic and proliferative factor in follicular cells from the rat ovary.