Determination of parishin, parishin B and parishin C in traditional Chinese medicinal formulas by micellar electrokinetic capillary chromatography
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文摘
Micellar electrokinetic capillary chromatographic methods were established for the determination of parishin, parishin B and parishin C in two kinds of the traditional Chinese medicinal formulas including Gastrodiae Rhizoma: Tsan-Siang-Tian-Ma-Tang and Ban-Shia-Bai-Ju-Tian-Ma-Tang. The samples were analyzed using 60-cm of uncoated capillary with various concentrations of sodium cholate and methanol as buffer, and detected at UV 222 nm. Regression equations were derived showing linear relationships (correlation coefficients: 0.9991–0.9997) between the peak-area ratios of each marker (parishin, parishin B and parishin C) to internal standard and concentration. The recoveries of parishin, parishin B and parishin C from the Chinese medicinal preparations were 102.3–102.7 % , 103.9–106.3 % and 97.8–104.0 % , respectively. The relative standard deviations of three marker constituents ranged between 1.46 and 3.08 % (intra-day), and 2.30 and 4.81 % (inter-day). The parishin, parishin B and parishin C contents measured 1.92–6.07 mg/g, 4.70–5.12 mg/g and 9.74–10.61 mg/g, respectively.