- 作者：Alireza Esteghamati ; Omid Khalilzadeh ; Zahra Mobarra ; Mehdi Anvari ; Maryam Tahvildari ; Hoda Mojazi Amiri ; Armin Rashidi ; Ghasem Solgi ; Kazem Parivar ; Behrouz Nikbin ; Aliakbar Amirzargar
- 关键词：Graves' disease ; Graves' ophthalmopathy ; CTLA-4 gene ; Polymorphism
- 刊名：European Journal of Internal Medicine
- 出版年：2009
- 出版时间：July 2009
- 年：2009
- 卷：20
- 期：4
- 页码：424-428
- 全文大小：149 K
BackgroundThe cytotoxic T lymphocyte associated antigen-4 (CTLA-4) gene, is one of the candidate genes for susceptibility to Graves' disease. This study aimed to investigate the association of Graves' disease and Graves' ophthalmopathy with polymorphisms at position + 49 in exon 1 and positions − 318 and − 1147 in the promoter region of CTLA-4 gene in Iranian patients.Methods
A total of 205 unrelated Iranian patients with Graves' disease who were referred to the outpatient endocrine clinic of a large university general hospital and 103 sex-matched healthy controls were included in this study. Venous blood was obtained, genomic DNA was extracted by a salting out method, and the polymorphisms at positions + 49, − 318 and − 1147 of the CTLA-4 gene were determined using the PCR-restriction fragment length polymorphism method (PCR-RFLP). Genotype and allele frequencies were determined.
Results
The frequency of the G allele at position + 49 was significantly higher in patients with Graves' disease than in the control group (27.1 % vs. 15.1 % , OR = 2.096, 95 % CI = 1.350–3.253 and p < 0.01). Significant trends were not seen for the other two polymorphisms studied. In patients with ophthalmopathy, the frequency of the G allele at position + 49 was higher than in those without ophthalmopathy (33.8 % vs. 20.0 % , OR = 2.043, 95 % CI = 1.304–3.202 and p < 0.01).
Conclusion
The results of this study suggest that the G allele at position + 49 in exon1 of the CTLA-4 gene is associated with Graves' disease and Graves' ophthalmopathy in Iranian patients.