The endocannabinoid system mediates aerobic exercise-induced antinociception in rats

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• 作者：Giovane Galdino ; Thiago R.L. Romero ; Jos茅 Felipe P. Silva ; Daniele C. Aguiar ; Ana Maria de Paula ; Jader S. Cruz ; Cosimo Parrella ; Fabiana Piscitelli ; Igor D. Duarte ; Vincenzo Di Marzo ; Andrea C. Perez
• 关键词：Endocannabinoids ; Cannabinoid receptors ; Aerobic exercise ; Pain ; Antinociception
• 刊名：Neuropharmacology
• 出版年：February, 2014
• 年：2014
• 卷：77
• 期：Complete
• 页码：313-324
• 全文大小：4136 K

文摘

Exercise-induced antinociception is widely described in the literature, but the mechanisms involved in this phenomenon are poorly understood. Systemic (s.c.) and central (i.t., i.c.v.) pretreatment with CB₁ and CB₂ cannabinoid receptor antagonists (AM251 and AM630) blocked the antinociception induced by an aerobic exercise (AE) protocol in both mechanical and thermal nociceptive tests. Western blot analysis revealed an increase and activation of CB₁ receptors in the rat brain, and immunofluorescence analysis demonstrated an increase of activation and expression of CB1 receptors in neurons of the periaqueductal gray matter (PAG) after exercise. Additionally, pretreatment (s.c., i.t. and i.c.v.) with endocannabinoid metabolizing enzyme inhibitors (MAFP and JZL184) and an anandamide reuptake inhibitor (VDM11) prolonged and intensified this antinociceptive effect. These results indicate that exercise could activate聽the endocannabinoid system, producing antinociception. Supporting this hypothesis, liquid-chromatography/mass-spectrometry measurements demonstrated that plasma levels of endocannabinoids (anandamide and 2-arachidonoylglycerol) and of anandamide-related mediators (palmitoylethanolamide and oleoylethanolamide) were increased after AE. Therefore, these results suggest that the endocannabinoid system mediates aerobic exercise-induced antinociception at peripheral and central levels.