Expression of Evc2 in craniofacial tissues and craniofacial bone defects in Evc2 knockout mouse
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- 作者：Mohammed K. Badria ; b ; Honghao Zhangc ; Yoshio Ohyamaa ; Sundharamani Venkitapathia ; Ahmed Alamoudia ; Nobuhiro Kamiyac ; d ; Haruko Takedae ; Manas Rayf ; Greg Scottf ; Takehito Tsujig ; Tetsuo Kuniedag ; Yuji Mishinac ; d ; f ; Yoshiyuki Mochidaa ; mochida@bu.edu" class="auth_mail" title="E-mail the corresponding author
- 关键词：EvC ; Ellis-van Creveld ; EVC2 ; Ellis-van Creveld 2 ; WT ; wild type ; KO ; knockout ; Het ; heterozygous ; IRES ; internal ribosomal entry site ; β-gal ; β-galactosidase ; IHC ; immunohistochemistry ; Hh ; hedgehog ; Smo ; Smoothened ; Ptch1 ; Patched1 ; SuFu ; suppressor of fused homolog ; E ; embryonic ; P ; postnatal ; PBS ; phosphate buffered saline ; PFA ; paraformaldehyde ; RT ; room temperature ; SOX9 ; sex determining region Y-box 9 ; Ig ; immunoglobulin ; KOH ; potassium hydroxide ; NCC ; neural crest cell ; LBN ; LIMBIN
- 刊名：Archives of Oral Biology
- 出版年：2016
- 出版时间：August 2016
- 年：2016
- 卷：68
- 期：Complete
- 页码：142-152
- 全文大小：5714 K
文摘
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Evc2 knockout mouse was generated by introducing a stop codon followed by IRES-LacZ.
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This IRES-LacZ cassette allows us to identify the Evc2-expressing cells.
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Evc2-expressing cells were mainly chondrocytes in the anterior viscerocranium.
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Endogenous EVC2 protein was observed in these corresponding cells.
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Evc2 deficiency showed abnormal craniofacial bones in the anterior viscerocranium.