B. melitensis can be transmitted via gastrointestinal, respiratory and reproductive tracts.

Intraperitoneal exposures of mouse model to live B. melitensis and its lipopolysaccharide elicited high antibody and cytokine immune response but resulted in decline of sex related hormones.

LPS extracted from B. melitensis induces immune response in the gastrointestinal, respiratory and reproductive tracts than the whole cell of the organism.

LPS stimulated significantly the innate and acquired immune system without significant systemic dysfunction, suggesting potentiality of the protective properties of this component as an alternative vaccine for brucellosis infection.

A validated experimental data of biological meaningful is further required to support the proposition that LPS may play a direct role in the sustainable control of B. melitensis infections in field conditions.