The effect of 2′,4′,7-trihydroxyisoflavone on ultraviolet-induced matrix metalloproteinases-1 expression in human skin fibroblasts

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• 作者：Hyung-In Moon and Jin Ho Chung
• 关键词：2′ ; 4′ ; 7-Trihydroxyisoflavone ; Ultraviolet radiation ; Matrix metalloproteinase-1 ; Human skin fibroblast
• 刊名：FEBS Letters
• 出版年：2006
• 出版时间：6 February 2006
• 年：2006
• 卷：580
• 期：3
• 页码：769-774
• 全文大小：183 K

文摘

UV-induced matrix metalloproteinases (MMPs) cause connective tissue damage and the skin to become wrinkled and aged. Here, we investigated the effect of 2′,4′,7-trihydroxyisoflavone (THF) on UV-induced MMP-1 expression in human skin fibroblasts (HSFs). We found that UV irradiation increases MMP-1 expression and that this is mediated by ERK and JNK activation, but not by p38 activation. Pretreatment of HSFs with 2′,4′,7-THF inhibited UV-induced MMP-1 expression in a dose-dependent manner, and also inhibited the UV-induced activations of ERK and JNK by inhibiting MEK1 and SEK1 activation, respectively. Moreover, inhibitions of ERK and JNK by 2′,4′,7-THF resulted in the decrease of c-Fos expression and c-Jun phosphorylation/expression induced by UV, respectively, which led to the inhibition of UV-induced AP-1 DNA binding activity. This inhibitory effect of 2′,4′,7-THF on MMP-1 was not mediated by an antioxidant effect. In conclusion, our results demonstrate that 2′,4′,7-THF can inhibit UV-induced MMP-1 expression by inhibiting the MEK1/ERK/c-Fos and SEK1/JNK/c-Jun pathways. Therefore, 2′,4′,7-THF is a potential agent for the prevention and treatment of skin aging.