Agave sisalana extract induces cell death in Aedes aegypti hemocytes increasing nitric oxide production

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• 作者：Louise Helena Guimarã ; es de Oliveira¹ ; Patricia Alexandria Paiva Silva de Sousa¹ ; Fabrine Felipe Hilario¹ ; Gabriel Joventino Nascimento¹ ; Joã ; o Paulo Saraiva Morais² ; Everaldo Paulo de Medeiros² ; Manoel Francisco de Sousa² ; Fabiola da Cruz Nunes¹ ; ^{fabiola@cbiotec.ufpb.br" class="auth_mail" title="E-mail the corresponding author}
• 关键词：Sisal ; Cell culture ; Mosquito ; Insecticide
• 刊名：Asian Pacific Journal of Tropical Biomedicine
• 出版年：2016
• 出版时间：May 2016
• 年：2016
• 卷：6
• 期：5
• 页码：396-399
• 全文大小：452 K

文摘

To investigate the effects of Agave sisalana (A. sisalana) extract on Aedes aegypti (Ae. aegypti) primary cell culture.

Methods

Cells of Ae. aegypti were exposed to different concentrations of A. sisalana crude extract (0.18–6.00 mg/mL) for 24 h. Then, the cells were labeled with propidium iodide and subjected to fluorescence microscopy to verify cell viability. In addition, nitric oxide production was measured.

Results

Results showed that cells exposed to 6 mg/mL of the crude extract presented a greater percentage of death when compared to control (73.8% ± 9.6% vs. 34.6% ± 9.6%). Furthermore, there was an increase in the nitric oxide production in cells exposed to 6 mg/mL of A. sisalana crude extract [(0.81 ± 0.08) μmol/L] compared to control group [(0.41 ± 0.18) μmol/L].

Conclusions

The results show that A. sisalana is cytotoxic to Ae. aegypti and may be used as raw material for new eco-friendly and inexpensive insecticides, since sisal industry discards the liquid waste for the extraction of plant fiber.