Microarray analysis identifies genes preferentially expressed in the lung schistosomulum of Schistosoma mansoni
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- 作者：Gary P. Dillon ; Theresa Feltwell ; Jason P. Skelton ; Peter D. Ashton ; Patricia S. Coulson ; Michael A Quail ; Nefeli Nikolaidou-Katsaridou ; R. Alan Wilson and Alasdair C. Ivens
- 关键词：Blood fluke ; Life cycle ; Transcriptome ; Secretion ; Membrane proteins ; Schistosoma
- 刊名：International Journal for Parasitology
- 出版年：2006
- 出版时间：January 2006
- 年：2006
- 卷：36
- 期：1
- 页码：1-8
- 全文大小：334 K
文摘
The lung schistosomulum of Schistosoma mansoni is a validated target of protective immunity elicited in vaccinated mice. To identify genes expressed at this stage we constructed a microarray, representing 3088 contigs and singlets, with cDNA derived from in vitro cultured larvae and used it to screen RNA from seven life-cycle stages. Clustering of genes by expression profile across the life cycle revealed a number of membrane, membrane-associated and secreted proteins up-regulated at the lung stage, that may represent potential immune targets. Two promising secreted molecules have homology to antigens with vaccine and/or immunomodulatory potential in other helminths.