Determination of glaucine in plasma and urine by high-performance liquid chromatography

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• 作者：Fels ; Jean-Pierre ; Lechat ; Patrick ; Rispe ; Raymond ; Cautreels ; Werner
• 刊名：Journal of Chromatography A
• 出版年：1998
• 出版时间：April, 1998
• 年：1998
• 卷：308
• 期：Complete
• 页码：273-281
• 全文大小：470 K

文摘

A sensitive method is described for the measurement of d-glaucine in pharmacokinetic studies using only 100-μl plasma samples or 200-μl urine samples. It requires a simple extraction clean-up on kieselguhr micro-columns and straight-phase high-performance liquid chromatography with fluorescence detection. Data on selectivity, sensitivity and precision demonstrate the reliability of this method. Its applicability is revealed by single and repeated oral administration pharmacokinetic studies in human subjects.