Estrogen deficiency increases osteoclastogenesis up-regulating T cells activity: A key mechanism in osteoporosis

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• 作者：Patrizia D'Amelio ; Anastasia Grimaldi ; Stefania Di Bella ; Stefano Z.M. Brianza ; Maria Angela Cristofaro ; Cristina Tamone ; Giuliana Giribaldi ; Daniela Ulliers ; Gian P. Pescarmona ; Giancarlo Isaia
• 关键词：Osteoclast ; Osteoporosis ; TNF ; RANKL ; T cell
• 刊名：Bone
• 出版年：2008
• 出版时间：July 2008
• 年：2008
• 卷：43
• 期：1
• 页码：92-100
• 全文大小：1685 K

文摘

Compelling evidences suggest that increased production of osteoclastogenic cytokines by activated T cells plays a relevant role in the bone loss induced by estrogen deficiency in the mouse. However, little information is available on the role of T cells in post-menopausal bone loss in humans. To investigate this issue we have assessed the production of cytokines involved in osteoclastogenesis (RANKL, TNF and OPG), in vitro osteoclast (OC) formation in pre and post-menopausal women, the latter with or without osteoporosis. We evaluated also OC precursors in peripheral blood and the ability of peripheral blood mononuclear cells to produce TNF in both basal and stimulated condition by flow cytometry in these subjects.