Meiotic spindle recovery is faster in vitrification of human oocytes compared to slow freezing

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• 作者：Patrizia Maria Ciotti ; Eleonora Porcu ; Leonardo Notarangelo ; Otello Magrini ; Antonia Bazzocchi ; Stefano Venturoli
• 关键词：Human oocyte ; meiotic spindle ; slow freezing ; vitrification ; temperature
• 刊名：Fertility and Sterility
• 出版年：2009
• 出版时间：June 2009
• 年：2009
• 卷：91
• 期：6
• 页码：2399-2407
• 全文大小：515 K

文摘

Objective

To investigate spindle behavior during and after slow freezing at room temperature (RT) and vitrification at different temperatures.

Design

Randomized, comparative study.

Setting

University hospital.

Patient(s)

Patients undergoing IVF treatment volunteered for the study and donated part of their supernumerary oocytes.

Intervention(s)

Metaphase II oocytes were divided into group A: slow freezing RT /thawing RT; group B: vitrification RT/warming RT; group C: vitrification RT/warming 37°C; and group D: vitrification 37°C/warming 37°C. Spindle presence was evaluated at each step of the four procedures and in culture.

Main Outcome Measure(s)

Cumulative spindle recovery rate comparing warming phase of the three vitrification groups and culture phase among the four groups.

Result(s)

During warming, the three vitrification groups showed a significantly fast spindle recovery rate compared to the thawing of the slow freezing group. A progressively significant fast cumulative recovery rate was observed in the three vitrification groups by increasing the number of phases at physiological temperature (hazard rate = 2.68; 95 % confidence interval 1.71–4.02).

Conclusion(s)

The present study demonstrates that spindle recovery is faster in vitrification than in slow freezing. These data support a possible protective effect of vitrification/warming at 37°C on the meiotic spindle structure and, therefore, on the subsequent clinical outcome of the procedure, although comparative clinical studies are needed.