A poly(o-aminophenol) modified electrode as an amperometric hydrogen peroxide biosensor
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- 作者：Valdé ; s Garcí ; a ; Marí ; a Ana ; Tuñ ; ó ; n Blanco ; Paulino ; Ivaska ; Ari
- 关键词：enzymatic biosensor ; hydrogen peroxide ; poly(o-aminophenol) films ; amperometric ; flow injection analysis
- 刊名：Electrochimica Acta
- 出版年：1998
- 出版时间：July 30, 1998
- 年：1998
- 卷：43
- 期：23
- 页码：3533-3539
- 全文大小：251 K
文摘
An enzymatic biosensor for hydrogen peroxide based on a horseradish peroxidase (HRP)-ferrocene carbon paste modified electrode and coated with a layer of electrochemically generated poly(o-aminophenol) is reported. A linear calibration curve is obtained over the range 1×10−8 M to 1×10−5 M. The biosensor responds to hydrogen peroxide in a few seconds and has a detection limit of 8.5×10−9 M. The response of the biosensor is diffusion controlled at low substrate concentrations. Flow injection assays of hydrogen peroxide at a sampling rate of 150 injections per hour with a relative standard deviation of 0.8 % (50 samples) are possible. Applicability of the sensor for measurement of hydrogen peroxide in real samples (milk) was demonstrated.