Hemo-acrylic polymers as catalyst for the oxidative dehalogenation of 2,4,6-trichlorophenol. Chloroperoxidase's mimic imprinting effects

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• 作者：Goretti Dí ; az-Dí ; az ; M. Carmen Blanco-Ló ; pez ; M. Jesú ; s Lobo-Casta&ntilde ; ó ; n ; Arturo J. Miranda-Ordieres ; Paulino Tu&ntilde ; ó ; n-Blanco ; ^{ptb@uniovi.es}
• 关键词：TCP ; 2 ; 4 ; 6-trichlorophenol ; CPO ; chloroperoxidase ; MA ; methacrylamide ; VPY ; 4-vinylpyridine ; EPA ; US Environmental Protection Agency ; EU ; European Union ; 4-C-3-MP ; 4-chloro-3-methylphenol ; 2 ; 4-DCP ; 2 ; 4-dichlorophenol ; 2 ; 4-DNP ; 2 ; 4-dinitrophenol ; DCQ ; 2 ; 6-
• 刊名：Journal of Molecular Catalysis A: Chemical
• 出版年：2012
• 出版时间：February, 2012
• 年：2012
• 卷：353-354
• 期：Complete
• 页码：117-121
• 全文大小：236 K

文摘

Acrylic polymers with catalytic activity for the oxidative degradation of 2,4,6-trichlorophenol (TCP) were developed. In order to mimic the active site of chloroperoxidase (CPO), chloro-iron(III)-protoporphyrin IX was used as the catalytic centre, and methacrylamide (MA) and 4-vinylpyridine (VPY) were used as the monomers that build up the active sites. Taking as basis 3:1 (w/w) acid:basic aminoacidic composition of CPO, three MA:VPY combinations were tested: one keeping the same ratio (3:1) i.e. 25 % VPY in the functional monomer mixture, one with lower content of the basic monomer (9:1) i.e. 10 % VPY, and one with higher concentration of it (1:1) i.e. 50 % VPY. Polymers synthesized with the lowest VPY content exhibited the highest catalytic efficiency, which was improved by the creation of specific TCP binding sites through molecular imprinting technology. In these way, synthetic enzymes with useful properties for analytical and bioremediation applications were obtained.