Mechanism of anti-hyperglycemic action of Vatairea macrocarpa (Leguminosae): Investigation in peripheral tissues

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• 作者：Paula Domingues Baviloni ; Maí ; sa Pavani dos Santos ; Gustavo Mitsuo Aiko ; Silvia Regina de Lima Reis ; Má ; rcia Queiroz Latorraca ; Virginia Claudia da Silva ; Ev ; ro Luiz Dall’ ; Oglio ; Paulo T
• 关键词：Diabetes mellitus ; Vatairea macrocarp ; Insulin signaling ; Gluconeogenesis ; Leguminosae ; Protein kinases
• 刊名：Journal of Ethnopharmacology
• 出版年：2010
• 出版时间：19 August 2010
• 年：2010
• 卷：131
• 期：1
• 页码：135-139
• 全文大小：254 K

文摘

Aim of the study

Previous studies in our laboratory have demonstrated that the treatment of diabetic rats during 21 days with V. macrocarpa stem-bark ethanolic extract (VmE), reduced glycemia, urinary glucose and urea, increased liver glycogen content and improved other parameters diabetes related. The objective of this study was to evaluate if the anti-hyperglicemic mechanisms of VmE could be caused by improvement in the insulin signaling pathway in the peripheral tissues (liver, adipose and skeletal muscle).

Material and methods

Streptozotocin-diabetic rats were separated into two groups: diabetic control (DC) and diabetic treated with VmE (DT) during 21 days. The alterations on the insulin signaling in liver, retroperitoneal adipose tissue (RET) and extensor digitorum longus (EDL) muscles were investigated through determination of insulin receptor (IR), protein kinase B/AKT content and AKT phosphorylation levels using Western blotting analysis. This same methodology was used to evaluate the phosphoenolpyruvate carboxykinase (PEPCK) levels in the liver from these animals.

Results

The treatment with the extract increased the content of IR and the basal phosphorylation of AKT in the three tissues. In the liver from diabetic treated group, the insulin-stimulated AKT phosphorylation was higher and the PEPCK protein levels were reduced.

Conclusions

Data from this work suggest that the anti-hyperglycemic activity of stem-bark extract of V. macrocarpa can occur through stimulation of insulin signaling pathways in peripheral tissues from diabetic rats, mainly in liver and adipose tissue, probably promoting increase in the glucose uptake and liver glycogen synthesis. The concomitant decreasing in hepatic PEPCK levels could be associated to inhibition of gluconeogenesis, which can also contribute to glycemia reduction.