Low-protein, high-carbohydrate diet increases glucose uptake and fatty acid synthesis in brown adipose tissue of rats

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• 作者：Sué ; lem Aparecida de Franç ; a ; M.Sc.^a ; Maí ; sa Pavani dos Santos ; M.Sc.^a ; Roger Viní ; cius Nunes Queiroz da Costa^a ; Mendalli Froelich ; M.Sc.^a ; Samyra Lopes Buzelle ; M.Sc.^b ; Valé ; ria Ernestâ ; nia Chaves ; Ph.D.^c ; Morenna Alana Giordani^a ; Mayara Peron Pereira ; M.Sc.^a ; Edson Moleta Colodel ; Ph.D.^d ; Clá ; udia Marlise Balbinotti Andrade ; Ph.D.^a ; Nair Honda Kawashita ; Ph.D.^a ; ^{Nairhonda@terra.com.br" class="auth_mail}
• 关键词：Low-protein ; high-carbohydrate diet ; Brown adipose tissue ; Fatty acids ; Glucose uptake ; Glycerol-3-phosphate
• 刊名：Nutrition
• 出版年：April, 2014
• 年：2014
• 卷：30
• 期：4
• 页码：473-480
• 全文大小：1243 K

文摘

Objective

The aim of this study was to evaluate glucose uptake and the contribution of glucose to fatty acid (FA) synthesis and the glycerol-3-phosphate (G3P) of triacylglycerol synthesis by interscapular brown adipose tissue (IBAT) of low-protein, high-carbohydrate (LPHC) diet-fed rats.

Methods

LPHC (6% protein; 74% carbohydrate) or control (17% protein; 63% carbohydrate) diets were administered to rats (鈭?00聽g) for 15聽d. Total FA and G3P synthesis and the synthesis of FA and G3P from glucose were evaluated in聽vivo by ³H₂O and ¹⁴C-glucose. Sympathetic neural contribution for FA synthesis was evaluated by comparing the synthesis in denervated (7聽d before) IBAT with that of the contralateral innervated side. The insulin signaling and 尾3 adrenergic receptor (尾3-AR) contents, as well as others, were determined by Western blot (Student's t test or analysis of variance; P聽鈮ぢ?.05).

Results

Total FA synthesis in IBAT was 133% higher in the LPHC group and was reduced 85% and 70% by denervation for the LPHC and control groups, respectively. Glucose uptake was 3.5-fold higher in the IBAT of LPHC rats than in that of the control rats, and the contribution of glucose to the total FA synthesis increased by 12% in control rats compared with 18% in LPHC rats. The LPHC diet increased the G3P generation from glucose by 270% and the insulin receptor content and the p-AKT insulin stimulation in IBAT by 120% and reduced the 尾3-AR content by 50%.

Conclusions

The LPHC diet stimulated glucose uptake, both the total rates and the rates derived from glucose-dependent FA and G3P synthesis, by increasing the insulin sensitivity and the sympathetic flux, despite a reduction in the 尾3-AR content.