The inhibition effect of Chlorpromazine against the β-lactam resistance of MRSA

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• 作者：Ryong Kong¹ ; Ok-Hwa Kang² ; Yun-Soo Seo² ; Su-Hyun Mun¹ ; Tian Zhou² ; Dong-Won Shin³ ; Dong-Yeul Kwon² ; ^{sssimi@wku.ac.kr" class="auth_mail" title="E-mail the corresponding author}
• 关键词：Chlorpromazine ; PBP2a ; β-Lactamase ; mecA ; blaZ ; MRSA
• 刊名：Asian Pacific Journal of Tropical Medicine
• 出版年：2016
• 出版时间：June 2016
• 年：2016
• 卷：9
• 期：6
• 页码：542-546
• 全文大小：834 K

文摘

To investigate the gene related to β-lactam resistance and to confirm the mechanism about a synergy effect between CPZ and β-lactam antibiotics.

Methods

To measure antibacterial activity, we performed a minimum inhibitory concentration (MIC) and synergy test. Transmission electron microscopy (TEM) was used in morphological analysis. To analyze gene expression, we conducted reverse transcriptase polymerase chain reaction (PCR).

Results

We confirmed a synergy effect between CPZ and β-lactam antibiotics. Furthermore, we observed that CPZ affect the cell envelope of MRSA by using TEM. At the gene level, CPZ reduced the expression of resistance genes.

Conclusions

Through this result, we hypothesize that a decrease of resistance factor expressions was caused by CPZ because it disrupts the activity of a sensor protein located in the cell membrane.