Phytoplankton IF-FISH: Species-specific labeling of cellular proteins by immunofluorescence (IF) with simultaneous species identification by fluorescence immunohybridization (FISH)
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- 作者：Megan E. Meeka ; b ; meekme@g.cofc.edu" class="auth_mail" title="E-mail the corresponding author ; Frances M. Van Dolaha ; b
- 关键词：PCNA ; proliferating cell nuclear antigen ; IF ; immunofluorescence ; FISH ; fluorescent in situ hybridization ; LSU ; large subunit ; rRNA ; ribosomal RNA ; PFA ; paraformaldehyde ; D-PBS ; DEPC-treated PBS ; D-PBST ; DEPC-treated PBS 0.5% Tween 20 ; FMO ; fluorescence minus one
- 刊名：Journal of Microbiological Methods
- 出版年：2016
- 出版时间：May 2016
- 年：2016
- 卷：124
- 期：Complete
- 页码：21-27
- 全文大小：903 K
文摘
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Optimized protocol for dual labeling of protein and rRNA in dinoflagellates
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Fluorescence labeling intensity of both probes was determined by flow cytometry
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PCNA labeling intensity did not clearly reflect growth rate in Karenia brevis
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The rRNA probe used was discovered to be not entirely species-specific
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This protocol is broadly applicable in phytoplankton research