Intrafibrillar silicified collagen scaffold modulates monocyte to promote cell homing, angiogenesis and bone regeneration

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• 作者：Jin-long Sun^a ; ¹ ; Kai Jiao^a ; ¹ ; Li-na Niu^a ; ^{niulina831013@126.com" class="auth_mail" title="E-mail the corresponding author} ; Yang Jiao^c ; Qun Song^a ; Li-juan Shen^a ; Franklin R. Tay^b ; ^{ftay@augusta.edu" class="auth_mail" title="E-mail the corresponding author} ; Ji-hua Chen^a ; ^{jhchen@fmmu.edu.cn" class="auth_mail" title="E-mail the corresponding author}
• 关键词：Angiogenesis ; Bone regeneration ; Cell homing ; Intrafibrillar silicification ; Immunomodulation ; Monocytes
• 刊名：Biomaterials
• 出版年：2017
• 出版时间：January 2017
• 年：2017
• 卷：113
• 期：Complete
• 页码：203-216
• 全文大小：4111 K

文摘

The immunomodulatory functions of monocytes are increasingly being recognized. Silicified collagen scaffolds (SCSs), produced by infiltrating collagen matrices with intrafibrillar amorphous silica, exhibit osteogenic and angiogenic potential and are promising candidates in tissue engineering. Here, we demonstrate that SCS promotes in situ bone regeneration and angiogenesis via monocyte immunomodulation. Increased numbers of TRAP-positive monocytes, nestin-positive bone marrow stromal cells (BMSCs) and CD31-positive and endomucin-positive new vessels can be identified from new bone formation regions in a murine calvarial defect model. In addition, sustained release of silicic acid by SCS stimulates differentiation of blood-derived monocytes into TRAP-positive cells, with increased expressions of SDF-1α, TGF-β1, VEGFa and PDGF-BB. These cytokines further promote homing of BMSCs and endothelial progenitor cells as well as neovascularization. Taken together, these novel findings indicate that SCSs possess the ability to enhance recruitment of progenitor cells and promote osteogenesis and angiogenesis by immunomodulation of monocytes.