文摘
The ability of 17伪-ethinylestradiol (EE2) to elevate vitellogenin levels were investigated in male flounder Platichthys flesus and vitellogenin concentrations in flounders from the Danish coastal environment were determined. Male flounders were exposed to 17伪-ethinylestradiol (EE2) via food or water. Average vitellogenin concentrations in the control fish ranged between 25 and 100聽ng聽mL鈭?/sup>1. Exposure to 5.1, 8.1 and 16.8聽ng EE2 L鈭?/sup>1 in water and 500 and 5000聽ng EE2 kg鈭?/sup>1 body weight (bw) every second day in the food increased the plasma vitellogenin concentration in a concentration and time dependent manner, whereas exposure to 2.7聽ng EE2 L鈭?/sup>1 in water for 21聽d and 5 and 50聽ng EE2 kg鈭?/sup>1 bw for 12 days in the food did not. EE2 could be detected in liver and testes (but not in muscle) after exposure to 8.1 and 16.8聽ng EE2 L鈭?/sup>1 in the water and 5000聽ng EE2 kg鈭?/sup>1 bw in the food; the highest concentration was 6聽ng g鈭?/sup>1 wet weight in liver. The majority of the male flounders collected from nine coastal Danish sites from 1999 to 2004 had vitellogenin concentrations below 100聽ng聽mL鈭?/sup>1, and only at two sites moderate estrogenic inputs were indicated.