Measurement of total and free docetaxel concentration in human plasma by ultra-performance liquid chromatography-tandem mass spectrometry
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- 作者：Raü ; l Rigo-Bonnina ; raulr@bellvitgehospital.cat" class="auth_mail" title="E-mail the corresponding author ; Sara Cobo-Sacristá ; nb ; Nú ; ria Gonzalo-Diegoc ; Helena Colomd ; Carmen Muñ ; oz-Sá ; nchezc ; Ander Urruticoecheae ; Catalina Faloe ; Pedro Alí ; aa
- 关键词：DCX ; docetaxel ; PK ; phamacokinetic ; PD ; pharmacodynamic ; HPLC ; high-performance liquid chromatography ; UV ; ultraviolet ; MS/MS ; tandem mass spectrometry ; UPLC ; ultra-performance liquid chromatography ; MTBE ; methyl t-butyl ether ; LC ; liquid chromatography ; MS ; mass spectrometer ; PCX ; paclitaxel ; QC ; quality control ; LLOQ ; lower limit of quantification ; ULOQ ; upper limit of quantification ; ESI ; electrospray ionization ; MRM ; multiple reaction monitoring ; m/z ; mass-to-charge ; EMA ; European Medicines
- 刊名：Journal of Pharmaceutical and Biomedical Analysis
- 出版年：2016
- 出版时间：5 January 2016
- 年：2016
- 卷：117
- 期：Complete
- 页码：140-149
- 全文大小：1060 K
文摘
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We developed UPLC–MS/MS methods to measure total and free docetaxel in plasma.
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Methods involved liquid–liquid extraction followed by dryness under N2 evaporation.
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To measure free docetaxel, sample preparation was preceded by ultrafiltration.
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Methods were fully validated using the EMA and CLSI-IFCC validation guidelines.
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These methods could be useful for PK studies in patients with breast cancer.