Clarification of animal cell cultures using an affinity aqueous two-phase systems.
A fused protein named LYTAG-Z was used as affinity ligand for the capture of human antibodies.
LYTAG shown affinity towards polyethylene glycol – PEG – and co-polymers of ethylene oxide and propylene oxide – EOPO – molecules.
Antibodies recovered with yield of 89% using a system composed 7% PEG3350 and 6% dextran.