A probe-free four-tube real-time PCR assay for simultaneous detection of twelve enteric viruses and bacteria
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- 作者：Chen Zhanga ; 1 ; Peihua Niua ; 1 ; Yanying Hongb ; 1 ; Ji Wanga ; Jingyun Zhangc ; Xuejun Maa ; maxj@ivdc.chinacdc.cn" class="auth_mail" title="E-mail the corresponding author ; tanwj@hotmail.com" class="auth_mail" title="E-mail the corresponding author
- 关键词：Diarrhea ; Real-time PCR ; Melting curve ; Virus ; Bacteria
- 刊名：Journal of Microbiological Methods
- 出版年：2015
- 出版时间：November 2015
- 年：2015
- 卷：118
- 期：Complete
- 页码：93-98
- 全文大小：571 K
文摘
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Twelve common diarrhea-associated pathogens can be simultaneously detected.
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Four-tube assay is sensitive and easy to operate without post-PCR gel electrophoresis step.
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The cost for the assay is approximately $5 per test for twelve pathogens.
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The whole process takes no more than 3 h and works with different real-time PCR machines.