Resveratrol inhibits firefly luciferase
详细信息 查看全文
- 作者：Adel Bakhtiarova ; Paul Taslimi ; Stephen J. Elliman ; Penelope A. Kosinski ; Brian Hubbard ; Michael Kavana ; Daniel M. Kemp
- 关键词：Resveratrol ; Firefly luciferase ; SirT1 ; Therapeutic ; Drug ; Cancer ; Neurodegeneration
- 刊名：Biochemical and Biophysical Research Communications
- 出版年：2006
- 出版时间：15 December 2006
- 年：2006
- 卷：351
- 期：2
- 页码：481-484
- 全文大小：230 K
文摘
The potential therapeutic value of resveratrol in age-related disease settings including cancer, diabetes, and Alzheimer’s has emerged from a rapidly growing body of experimental evidence. Protection from oxidative stress appears to be a common feature of resveratrol that may be mediated through SirT1, though more specific molecular mechanisms by which resveratrol mediates its effects remain unclear. This has prompted an upsurge in cell-based mechanistic studies, often incorporating reporter assays for pathway elucidation in response to resveratrol treatment. Here, we report that resveratrol potently inhibits firefly luciferase with a Ki value of 2 μM, and caution that this confounding element may lead to compromised data interpretation.