Bile deficiency induces changes in intestinal glucose absorption in mice

详细信息    查看全文

• 作者：Yehui Du ; MB^a ; ^b ; Hao Chen ; MM^a ; ^b ; Zefeng Xuan ; MM^a ; ^b ; Wenfeng Song ; BS^a ; ^b ; Liangjie Hong ; BS^a ; ^b ; Danjing Guo ; BS^a ; ^b ; Hongchun Li ; MM^c ; Biguang Tuo ; MD ; PhD^d ; Shusen Zheng ; MD ; PhD^a ; ^b ; Penghong Song ; PhD^a ; ^b ; ^{songpenghong@zju.edu.cn" class="auth_mail" title="E-mail the corresponding author}
• 刊名：Surgery
• 出版年：2016
• 出版时间：December 2016
• 年：2016
• 卷：160
• 期：6
• 页码：1496-1507
• 全文大小：3319 K

文摘

Biliary tract obstruction is a common clinical problem. In this study, we attempted to understand the change in intestinal glucose absorption after biliary tract obstruction.

Methods

Experimental models of murine biliary duct ligation and external biliary drainage were established. Murine intestinal mucosal glucose absorption was examined with Ussing chambers according to the increase in the short-circuit current in vitro and blood glucose measurement after oral glucose in vivo. The protein expression of the sodium-glucose cotransporter (SGLT1) and the facilitated glucose transporter, member 2 (GLUT2) was analyzed by Western blot and immunohistochemistry.

Results

The results from Ussing chamber experiments showed that duodenal mucosal glucose absorption levels were significantly higher in biliary duct ligation and biliary drainage mice than those in normal control mice at 1 and 2 weeks after the operation. Gastrointestinal bile acid administration almost reversed the elevated duodenal mucosal glucose absorption to the normal level in biliary drainage mice. The results from the experiments in vivo further confirmed that the glucose absorption increased in biliary duct ligation and biliary drainage mice. The protein expression levels of SGLT1 in the duodenal mucosae of both biliary duct ligation and biliary drainage mice were markedly higher than those in control mice, and the protein expression of GLUT2 was not significantly altered, compared with control mice.

Conclusion

Bile deficiency in the intestine upregulates the expression of intestinal mucosal SGLT1 and enhances intestinal mucosal glucose absorption capacity, which contributes to the understanding of intestinal physiologic function for patients with biliary duct obstruction and external biliary drainage.