In-tip nanoreactors for cancer cells proteome profiling
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- 作者：Ling Yana ; Liang Qiaoa ; Ji Jia ; Yixin Lia ; Xuefei Yina ; Ling Lina ; Xiaohui Liua ; Jun Yaoa ; Yi Wanga ; Bin Liub ; Kun Qianb ; Baohong Liua ; Pengyuan Yanga ; pyyang@fudan.edu.cn" class="auth_mail" title="E-mail the corresponding author
- 关键词：Nanoporous materials ; Micropipette tips ; Proteolysis ; Mass spectrometry ; Profiling ; Cancer cells
- 刊名：Analytica Chimica Acta
- 出版年：2017
- 出版时间：1 January 2017
- 年：2017
- 卷：949
- 期：Complete
- 页码：43-52
- 全文大小：2314 K
文摘
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Increased number of identified proteins compared to the 12 h in-solution digestion.
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Enhanced proteolytic performance towards a group of proteins with specific pI.
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Desirable throughput consuming ∼6 min for proteolysis of bio-samples.
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Automated process in the sample pretreatment for online MS analysis.
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Long term stability after two months storage, reusability with little memory effect.