Distinct oxidative cleavage and modification of bovine [Cu- Zn]-SOD by an ascorbic acid/Cu(II) system: Identification of novel copper binding site on SOD molecule
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- 作者：Hiroshi Ueharaa ; Shen Luoa ; Baikuntha Aryala ; Rodney L. Levineb ; V. Ashutosh Raoa ; ashutosh.rao@fda.hhs.gov" class="auth_mail" title="E-mail the corresponding author
- 关键词：bSOD1 ; bovine [Cu&ndash ; Zn]-superoxide dismutase ; MCO ; metal catalyzed oxidation ; ALS ; amyotrophic lateral sclerosis ; ROS ; reactive oxygen species ; Asc ; sodium ascorbate ; LC&ndash ; ESI-Q-TOF-MS ; liquid chromatography&ndash ; electrospray ionization-quadrupole-time of flight-mass spectrometry ; DTPA ; diethylene-triamine-penta-acetic acid ; CBB ; Coomassie Brilliant Blue
- 刊名：Free Radical Biology and Medicine
- 出版年：2016
- 出版时间：May 2016
- 年：2016
- 卷：94
- 期：Complete
- 页码：161-173
- 全文大小：2889 K
文摘
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Ascorbate/Cu(II) mediates cleavage of bSOD1 distinct from peroxide-mediated cleavage.
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Ascorbate/ Cu(II)-mediated specific cleavage of bSOD1 is inhibited by Fe(II).
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Mass spectrometry analyses demonstrated a novel peptide bond cleavage at Gly31.
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A new His-19 coordinated binding site is proposed for exogenous copper.