Heterologous expression of the human Phosphoenol Pyruvate Carboxykinase (hPEPCK-M) improves hydrogen and ethanol synthesis in the Escherichia coli dcuD mutant when grown in a glycerol-based medium

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• 作者：Antonio Valle^a ; ^{antonio.valle@uca.es} ; ^{antoniovalle82@gmail.com} ; Gema Cabrera^b ; Domingo Cantero^b ; Jorge Bolivar^a ; ^{jorge.bolivar@uca.es}
• 关键词：Ethanol ; Glycerol fermentation ; hPEPCK-M ; Hydrogen
• 刊名：New Biotechnology
• 出版年：2017
• 出版时间：25 March 2017
• 年：2017
• 卷：35
• 期：Complete
• 页码：1-12
• 全文大小：2021 K
• 卷排序：35

文摘

A metabolic engineering strategy is necessary to enhance the production of hydrogen and ethanol in E. coli through the redirection of C4 metabolites. The human PEPCK-M enzyme was selected for expression in E. coli according to kinetic parameters and type of regulation. The physiological expression of h-PEPCK-M in the dcuD E. coli mutant strain enhances hydrogen and ethanol production with respect to E. coli Pck under low pressure conditions.