Modeling rotavirus infection and antiviral therapy using primary intestinal organoids
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- 作者：Yuebang Yina ; Marcel Bijveldsa ; Wen Danga ; Lei Xua ; Annemiek A. van der Eijkb ; Karen Knippingc ; Nesrin Tuysuzd ; Johanna F. Dekkerse ; Yijin Wanga ; Jeroen de Jongef ; Dave Sprengersa ; Luc J.W. van der Laanf ; Jeffrey M. Beekmane ; Derk ten Berged ; Herold J. Metselaara ; Hugo de Jongea ; Marion P.G. Koopmansb ; Maikel P. Peppelenboscha ; Qiuwei Pana ; q.pan@erasmusmc.nl" class="auth_mail" title="E-mail the corresponding author
- 关键词：IFN-α ; interferon-alpha ; dsRNA ; double stranded RNA ; CF ; cystic fibrosis ; IHC ; immunohistochemistry ; qRT-PCR ; quantitative real-time polymerase chain reaction ; GAPDH ; glyceraldehyde 3-phosphate dehydrogenase ; VP ; viral protein ; ISG ; interferon-stimulated gene ; IRF ; interferon regulatory factor ; IFITM ; interferon-induced transmembrane protein ; STAT ; signal transducer and activator of transcription ; PKR ; protein kinase R ; HCV ; hepatitis C virus ; DMEM ; Dulbecco&rsquo ; s modified Eagle&rsquo ; s medium
- 刊名：Antiviral Research
- 出版年：2015
- 出版时间：November 2015
- 年：2015
- 卷：123
- 期：Complete
- 页码：120-131
- 全文大小：2319 K
文摘
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3D-based primary intestinal organoids support the infection of both experimental and patient-derived rotavirus strains.
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Rotavirus infection provokes the induction of interferon-stimulated genes in organoids.
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Interferon-alpha and ribavirin inhibit rotavirus replication in organoids.
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Organoids infected with patient rotavirus can be potentially utilized for personalized evaluation of antivirals.