Sequence characterized amplified region (SCAR) marker (CRF-S870) associated with a restoration-of-fertility (Rf) locus was validated.
This dominant (CRF-S870) marker was then used in marker-assisted backcrossing (MAB) to transfer the Rf allele from hot pepper (AVPP9905) to sweet pepper genotypes.
The normal fertility restoration ability of 21 BC4F2 putative restorer sweet pepper plants was confirmed.