Non-Enveloped Virus Entry: Structural Determinants and Mechanism of Functioning of a Viral Lytic Peptide
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- 作者：Saumya Bajaj ; Debajit Dey ; Rohan Bhukar ; Mohit Kumar ; Manidipa Banerjee ; mbanerjee@bioschool.iitd.ac.in" class="auth_mail" title="E-mail the corresponding author
- 关键词：FHV ; Flock House Virus ; γ1 ; N-terminal region of gamma ; iASU ; icosahedral asymmetric unit ; cryo-EM ; cryo-electron microscopy ; VLPs ; virus-like particles ; MD ; molecular dynamics ; WT ; wild-type ; DOPC ; 1 ; 2-dioleoyl-sn-glycero-3-phosphocholine ; SulfoB ; sulforhodamine B
- 刊名：Journal of Molecular Biology
- 出版年：2016
- 出版时间：28 August 2016
- 年：2016
- 卷：428
- 期：17
- 页码：3540-3556
- 全文大小：3046 K
文摘
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Combinatorial study of biochemical assays, cryo-electron microscopy, and molecular dynamics simulations.
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The alpha-helical hairpin motif in the gamma peptide of Flock House Virus was disrupted.
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This causes loss of membrane penetration functionality, while structural positioning is unaffected.
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MD simulation shows membrane disruption facilitated by pentameric unit of full-length gamma.