A simple sheathless CE-MS interface with a sub-micrometer electrical contact fracture for sensitive analysis of peptide and protein samples
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- 作者：Tam T.T.N. Nguyen ; Nickolaj J. Petersen ; Nickolaj.Petersen@sund.ku.dk" class="auth_mail" title="E-mail the corresponding author ; Kasper D. Rand ; Kasper.Rand@sund.ku.dk" class="auth_mail" title="E-mail the corresponding author
- 关键词：Sheathless CE-MS interface ; Sub-micrometer fracture ; Protein/peptide analysis ; Protein ESI-MS ; Peptide mapping ; Native MS
- 刊名：Analytica Chimica Acta
- 出版年：2016
- 出版时间：14 September 2016
- 年：2016
- 卷：936
- 期：Complete
- 页码：157-167
- 全文大小：1933 K
文摘
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We develop a sheathless CE-MS interface with a sub-micron fracture directly in the capillary for electrical contact.
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The simple design facilitates a stable ESI spray, a low (attomol) detection limit and well-resolved CE separation.
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We apply the CE-MS setup for analysis of complex samples including mixtures of peptides and proteins.
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We demonstrate that the CE-MS method can also facilitate native MS applications.