A sensitive and practical RP-HPLC-FLD for determination of the low neuroactive amino acid levels in body fluids and its application in depression
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- 作者：Juan-Li Wua ; Si-Yang Yua ; Shi-Hua Wub ; Ai-Min Baoa ; baoaimin@zju.edu.cn" class="auth_mail" title="E-mail the corresponding author
- 关键词：CSF ; cerebrospinal fluid ; AAs ; amino acids ; HPLC ; high performance liquid chromatography ; FLD ; fluorescence detection ; OPA ; o-phthaldialdehyde ; MPA ; β-mercaptopropionic acid ; THF ; tetrahydrofuran ; TEA ; triethanolamine ; MDD ; major depressive disorder ; HClO4 ; perchloric acid
- 刊名：Neuroscience Letters
- 出版年：2016
- 出版时间：11 March 2016
- 年：2016
- 卷：616
- 期：Complete
- 页码：32-37
- 全文大小：1081 K
文摘
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Detection of amino acids in the cerebrospinal fluid is also of clinical importance.
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HPLC measurements of CSF amino acids (AAs) levels is challenging due to low levels.
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Improved protocol for HPLC detection for CSF-AAs measurement is presented.
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Improvement was achieved by modifying pH, derivatization and mobile phases.
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The improved protocol is applicable for detection of other neuroactive AAs.