Anammox metabolism was inhibited by the addition of 487.5 mg·L−1 COD.
A COD/N ratio of 5.0 (COD of 300 mg·L−1) did not affect nitrogen removal.
Anaerobic effluent changed bacterial community structure and diversity.
Anammox and denitrifying bacteria were both found in the reactor.
Anammox can be applied to remove nitrogen from pre-treated municipal wastewater.