- 作者：T.O. Ajiboye ; ajiboyeyong@yahoo.com" class="auth_mail" title="E-mail the corresponding author ; H. Aliyu ; M.A. Tanimu ; R.M. Muhammad ; O.B. Ibitoye
- 关键词：HF ; High fructose diet ; BW ; Body weight ; TC ; Total cholesterol ; TAG ; Triglycerides ; LDLc ; Low density lipoprotein cholesterol ; VLDLc ; Very low density lipoprotein cholesterol ; HDLc ; High density lipoprotein cholesterol ; SOD ; Superoxide dismutase ; CAT ; Catalase ; GSH-Px ; Glutathione peroxidase ; GSH-Red ; Glutathione reductase ; Glc 6-PD ; Glucose 6-phosphate dehydrogenase ; ROS ; Reactive oxygen species ; DNA ; Deoxyribonucleic acid ; SEM ; Standard error of mean ; CD ; conjugated dienes ; LH ; Lipid hydropero
- 刊名：Journal of Ethnopharmacology
- 出版年：2016
- 出版时间：4 November 2016
- 年：2016
- 卷：192
- 期：Complete
- 页码：471-479
- 全文大小：1485 K
Methods
Seventy male rats were randomized into seven groups. All rats were fed with high-fructose diet for 9 weeks except groups A and C rats, which received control diet. In addition to the diet treatment, groups A and B rats received distilled water for 3 weeks starting from the seventh week of the experimental period. Rats in groups C–F orally received 400, 100, 200 and 400 mg/kg body weight of aqueous leaf extract of D. cumminsii respectively, while group G received 300 mg/kg bodyweight of metformin for 3 weeks starting from the seventh week.
Results
There was significant (p<0.05) reduction in high-fructose diet-mediated increase in body weight, body mass index, abdominal circumference, blood glucose, insulin, leptin and insulin resistance by aqueous leaf extract of D. cumminsii. Conversely, high-fructose diet-mediated decrease in adiponectin was reversed by the extract. Increased levels of cholesterol, triglycerides, low-density lipoprotein cholesterol, very low-density lipoprotein cholesterol, atherogenic index, cardiac index and coronary artery index were significantly lowered by the extract, while high-fructose diet mediated decrease in high-density lipoprotein cholesterol was increased by the extract. Tumour necrosis factor-α, interleukin-6 and interleukin-8 levels increased significantly in high-fructose diet-fed rats, which were significantly reversed by the extract. High-fructose mediated-decrease in superoxide dismutase, catalase, glutathione peroxidase, glutathione reductase, glucose 6-phosphate dehydrogenase and glutathione reduced were significantly reversed by aqueous leaf extract of D. cumminsii. Conversely, elevated levels of malondialdehyde, conjugated dienes, lipid hydroperoxides, protein carbonyl and fragmented DNA were significantly lowered by the extract.
Conclusion
Data generated in this study further laid credence to the hypoglycemic activity of aqueous leaf extract of D. cumminsii as evident from the reversal of hyperglycemia, insulin resistance, dyslipidemia, inflammation and oxidative stress in high-fructose diet-induced metabolic syndrome rats.