Green Tea Polyphenol Prevents Diabetic Rats From Acute Kidney Injury After Cardiopulmonary Bypass

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• 作者：Masaki Funamoto ; MD^a ; Hidetoshi Masumoto ; MD ; PhD^a ; Koji Takaori ; MD^b ; Tomofumi Taki ; MD^a ; Shuji Setozaki ; MD^a ; Kazuhiro Yamazaki ; MD ; PhD^a ; Kenji Minakata ; MD ; PhD^a ; Tadashi Ikeda ; MD ; PhD^a ; ^{ike@kuhp.kyoto-u.ac.jp" class="auth_mail" title="E-mail the corresponding author} ; Suong-Hyu Hyon ; PhD^c ; Ryuzo Sakata ; MD ; PhD^a
• 关键词：8-OHdG ; 8-hydroxy-2&prime ; -deoxyguanosine ; AKI ; acute kidney injury ; BG ; blood glucose ; CPB ; cardiopulmonary bypass ; DM ; diabetes mellitus ; EGCG ; epigallocatechin-3-gallate ; HO-1 ; hemoxygenase-1 ; HR ; heart rate ; Kim-1 ; kidney injury molecule-1 ; MAP ; mean arterial pressure ; mRNA ; messenger RNA ; NGAL ; neutrophil gelatinase-associated lipocalin ; Pao2 ; partial pressure of arterial oxygen ; PAS ; periodic acid-Schiff ; RT ; rectal temperature ; T-24h ; 24 hours after termination of CPB ; T-cpb ; 15 minutes a
• 刊名：The Annals of Thoracic Surgery
• 出版年：2016
• 出版时间：April 2016
• 年：2016
• 卷：101
• 期：4
• 页码：1507-1513
• 全文大小：1483 K

文摘

Acute kidney injury (AKI) is a common complication accompanying cardiopulmonary bypass (CPB) and is independently associated with increased morbidity and death. Diabetes mellitus increases the risk for AKI after CPB. Epigallocatechin-3-gallate (EGCG) is a major component of the polyphenolic fraction of green tea, which possesses cardioprotective activities, as previously reported. We hypothesized that EGCG also possesses a renoprotective effect through its diverse biochemical properties and assessed the effect on renal function after CPB for diabetic rats.

Methods

Goto-Kakizaki rats developing type 2 diabetes mellitus were randomly assigned to one of the following groups: sham (n = 10), CPB (CPB alone, n = 9), or EGCG (CPB + EGCG, n = 10). CPB was conducted for 30 minutes at a flow rate of 100 mL/kg/min in the CPB and EGCG groups. Rats assigned to the EGCG group were administrated EGCG solution orally for 2 weeks before CPB. We evaluated renal biochemical or histologic changes at 24 hours after CPB.

Results

Compared with the CPB group, the EGCG group exhibited milder tubular injury histologically (p < 0.0001) and reduced expression of kidney injury molecule-1, a biomarker for renal tubular injury (p < 0.0001) and 8-hydroxy-2′-deoxyguanosine (p < 0.01), indicating attenuated oxidant stress.

Conclusions

Preoperative oral administration of EGCG ameliorates AKI in a CPB model of diabetic rats through antioxidative properties. This simple method could be applied in a clinical setting as a prophylactic renal protection against AKI after CPB, especially for high-risk patients with diabetes mellitus.